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KMID : 0545120140240091291
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Journal of Microbiology and Biotechnology
2014 Volume.24 No. 9 p.1291 ~ p.1299
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Development of Two-Component Nanorod Complex for Dual-Fluorescence Imaging and siRNA Delivery
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Choi Jin-Ha
Oh Byung-Keun
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Abstract
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Recently, multifunctional nanomaterials have been developed as nanotherapeutic agents for cellular imaging and targeted cancer treatment because of their ease of synthesis and low cytotoxicity. In this study, we developed a multifunctional, two-component nanorod consisting of gold (Au) and nickel (Ni) blocks that enables dual-fluorescence imaging and the targeted delivery of small interfering RNA (siRNA) to improve cancer treatment. Fluorescein isothiocyanate-labeled luteinizing hormone-releasing hormone (LHRH) peptides were attached to the surface of a Ni block via a histidine-tagged LHRH interaction to specifically bind to a breast cancer cell line, MCF-7. The Au block was modified with TAMRA-labeled thiolated siRNA in order to knock down the vascular endothelial growth factor protein to inhibit cancer growth. These two-component nanorods actively targeted and internalized into MCF-7 cells to induce apoptosis through RNA interference. This study demonstrates the feasibility of using two-component nanorods as a potential theranostic in breast cancer treatment, with capabilities in dual imaging and targeted gene delivery.
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KEYWORD
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Two-component nanorod, siRNA, Cancer treatment, targeting delivery, dual imaging
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